A new NEDD8-ligating system for cullin-4A

Genes Dev. 1998 Aug 1;12(15):2263-8. doi: 10.1101/gad.12.15.2263.

Abstract

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cullin Proteins*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Humans
  • In Vitro Techniques
  • Ligases / genetics
  • Ligases / metabolism
  • Molecular Sequence Data
  • NEDD8 Protein
  • Rabbits
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Ubiquitin-Activating Enzymes
  • Ubiquitin-Protein Ligases
  • Ubiquitins / genetics
  • Ubiquitins / metabolism*

Substances

  • Cell Cycle Proteins
  • Cullin 1
  • Cullin Proteins
  • DNA-Binding Proteins
  • NEDD8 Protein
  • NEDD8 protein, human
  • Saccharomyces cerevisiae Proteins
  • UBC12 protein, S cerevisiae
  • Ubiquitins
  • Ubiquitin-Protein Ligases
  • Ligases
  • Ubiquitin-Activating Enzymes
  • NAE protein, human

Associated data

  • GENBANK/AB012190
  • GENBANK/AB012191
  • GENBANK/AB012193
  • GENBANK/D23662