Abstract
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30, SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line, Transformed
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DNA-Binding Proteins / physiology*
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Histone Deacetylases / genetics
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Histone Deacetylases / physiology*
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Homeodomain Proteins / physiology*
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Humans
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Kidney
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Macromolecular Substances
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Mice
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Models, Genetic
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Molecular Sequence Data
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Multiprotein Complexes
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Nuclear Proteins / physiology*
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Nuclear Receptor Co-Repressor 1
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Receptors, Estrogen / antagonists & inhibitors
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Receptors, Retinoic Acid / physiology
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Receptors, Thyroid Hormone / physiology
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Repressor Proteins / physiology*
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Saccharomyces cerevisiae Proteins*
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Transcription Factor Pit-1
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Transcription Factors / physiology*
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Transcription, Genetic*
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Transfection
Substances
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DNA-Binding Proteins
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Homeodomain Proteins
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Macromolecular Substances
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Multiprotein Complexes
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NCOR1 protein, human
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Ncor1 protein, mouse
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Nuclear Proteins
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Nuclear Receptor Co-Repressor 1
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POU1F1 protein, human
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Pit1 protein, mouse
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Receptors, Estrogen
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Receptors, Retinoic Acid
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Receptors, Thyroid Hormone
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Repressor Proteins
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SAP30 protein, human
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SIN3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Sap30 protein, mouse
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Transcription Factor Pit-1
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Transcription Factors
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Histone Deacetylases