Circularization of mRNA by eukaryotic translation initiation factors

Mol Cell. 1998 Jul;2(1):135-40. doi: 10.1016/s1097-2765(00)80122-7.

Abstract

Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor eIF4G. Here, we demonstrate the reconstitution of an eIF4E/eIF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Eukaryotic Initiation Factor-4E
  • Eukaryotic Initiation Factor-4G
  • Fungal Proteins / metabolism
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Macromolecular Substances
  • Microscopy, Atomic Force
  • Nucleic Acid Conformation*
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Peptide Initiation Factors / genetics
  • Peptide Initiation Factors / metabolism*
  • Peptide Initiation Factors / ultrastructure
  • Poly(A)-Binding Proteins
  • Protein Biosynthesis
  • RNA / biosynthesis*
  • RNA / ultrastructure
  • RNA, Circular
  • RNA, Fungal / chemistry
  • RNA, Fungal / metabolism*
  • RNA, Fungal / ultrastructure
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism*
  • RNA, Messenger / ultrastructure
  • RNA-Binding Proteins / metabolism*
  • RNA-Binding Proteins / ultrastructure
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins

Substances

  • EIF4G1 protein, human
  • Eukaryotic Initiation Factor-4E
  • Eukaryotic Initiation Factor-4G
  • Fungal Proteins
  • Macromolecular Substances
  • Peptide Fragments
  • Peptide Initiation Factors
  • Poly(A)-Binding Proteins
  • RNA, Circular
  • RNA, Fungal
  • RNA, Messenger
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • TIF4631 protein, S cerevisiae
  • RNA
  • Glutathione Transferase