Expression of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) and beta-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae

Biosci Biotechnol Biochem. 1998 Aug;62(8):1615-8. doi: 10.1271/bbb.62.1615.

Abstract

A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the recombinant BGL1 gave one band (180 kDa) by SDS-PAGE. The recombinant CBHI and BGL1 had the same enzymatical properties as the native enzyme except for the specific activity toward cellulosic substrates. By the combination of three different types of cellulases, FI-CMCase, CBHI, and BGL1, we could hydrolyze Avicel up to 59% under our experimental conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology*
  • Aspergillus / genetics
  • Cellulase / biosynthesis*
  • Cellulase / chemistry
  • Cellulase / genetics
  • Cellulose / chemistry
  • Cellulose 1,4-beta-Cellobiosidase
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Fungal*
  • Genetic Vectors
  • Molecular Weight
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Substrate Specificity
  • beta-Glucosidase / biosynthesis*
  • beta-Glucosidase / chemistry
  • beta-Glucosidase / genetics

Substances

  • Recombinant Proteins
  • Cellulose
  • beta-Glucosidase
  • Cellulase
  • Cellulose 1,4-beta-Cellobiosidase