Cloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus Neocallimastix frontalis MCH3

Biosci Biotechnol Biochem. 1998 Sep;62(9):1795-8. doi: 10.1271/bbb.62.1795.

Abstract

A cDNA clone encoding an endo-1,4-beta-glucanase from a rumen fungus, Neocallimastix frontalis MCH3, was isolated. The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome. The enzyme expressed in Escherichia coli showed the highest activity against carboxymethylcellulose at 40 degrees C and pH 8.5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anaerobiosis
  • Animals
  • Base Sequence
  • Cellulase / genetics*
  • Chytridiomycota / enzymology*
  • Chytridiomycota / genetics
  • Cloning, Molecular
  • DNA, Fungal
  • Gene Expression
  • Molecular Sequence Data
  • Rumen / microbiology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • CelA endoglucanase
  • Cellulase

Associated data

  • GENBANK/U38843