The plasminogen activator inhibitor-2 gene is not required for normal murine development or survival

Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):686-91. doi: 10.1073/pnas.96.2.686.

Abstract

Plasminogen activator inhibitor-2 (PAI-2), a member of the serpin gene family, is thought to serve as a primary regulator of plasminogen activation in the extravascular compartment. High levels of PAI-2 are found in keratinocytes, monocytes, and the human trophoblast, the latter suggesting a role in placental maintenance or embryo development. The primarily intracellular distribution of PAI-2 also may indicate a unique regulatory role in a protease-dependent cellular process such as apoptosis. To examine the potential functions of PAI-2 in vivo, we generated PAI-2-deficient mice by gene targeting in embryonic stem cells. Homozygous PAI-2-deficient mice exhibited normal development, survival, and fertility and were also indistinguishable from normal controls in response to a bacterial infectious challenge or endotoxin infusion. No differences in monocyte recruitment into the peritoneum were observed after thioglycollate injection. Epidermal wound healing was equivalent among PAI-2 -/- null and control mice. Finally, crossing PAI-2 -/- with PAI-1 -/- mice to generate animals deficient in both plasminogen activator inhibitors failed to uncover an overlap in function between these two related proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Southern
  • Gene Targeting / methods
  • Genotype
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Monocytes / immunology
  • Phenotype
  • Plasminogen Activator Inhibitor 1 / genetics
  • Plasminogen Activator Inhibitor 1 / metabolism
  • Plasminogen Activator Inhibitor 2 / genetics*
  • Plasminogen Activator Inhibitor 2 / metabolism
  • Stem Cells / metabolism
  • Wound Healing / genetics

Substances

  • Plasminogen Activator Inhibitor 1
  • Plasminogen Activator Inhibitor 2

Associated data

  • GENBANK/AF069683
  • GENBANK/AF069684
  • GENBANK/AF069685
  • GENBANK/AF069686
  • GENBANK/AF069687
  • GENBANK/AF069688
  • GENBANK/AF069689
  • GENBANK/AF069690
  • GENBANK/AF069691
  • GENBANK/AF069692
  • GENBANK/AF069693
  • GENBANK/AF069694
  • GENBANK/AF069695