Assignment of the melatonin-related receptor to human chromosome X (GPR50) and mouse chromosome X (Gpr50)

Genomics. 1999 Jan 15;55(2):248-51. doi: 10.1006/geno.1998.5661.

Abstract

Recent efforts to clone further members of the melatonin receptor family have led to the identification of a novel G-protein-coupled receptor in human pituitary. This receptor, referred to as H9, is clearly related to high-affinity melatonin receptors yet unable to bind this hormone. We now report the cloning and expression of the cDNA encoding the H9 receptor in mice. The mouse clone encodes a protein of 591 amino acids that shares 74% amino acid identity with the human receptor and is unable to bind 2-[125I]iodomelatonin when transiently expressed in COS-7 cells. We also determined the chromosome loci of the human and mouse H9 receptor genes. Both genes were found to be X-linked: radiation hybrid mapping revealed that the human H9 gene (GPR50) is localized to Xq28. The mouse gene (Gpr50) was determined to lie in the proximal portion of chromosome X by means of interspecific backcross analysis. These loci might be relevant to genetically based neuroendocrine disorders.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, G-Protein-Coupled*
  • Sequence Analysis, DNA
  • X Chromosome*

Substances

  • DNA, Complementary
  • GPR50 protein, human
  • Gpr50 receptor, mouse
  • Nerve Tissue Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled

Associated data

  • GENBANK/AF065145
  • GENBANK/U52219