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Highly efficient, large volume flow electroporation.
Li LH, Shivakumar R, Feller S, Allen C, Weiss JM, Dzekunov S, Singh V, Holaday J, Fratantoni J, Liu LN. Li LH, et al. Among authors: shivakumar r. Technol Cancer Res Treat. 2002 Oct;1(5):341-50. doi: 10.1177/153303460200100504. Technol Cancer Res Treat. 2002. PMID: 12625759 Free article.
CD38 knockout natural killer cells expressing an affinity optimized CD38 chimeric antigen receptor successfully target acute myeloid leukemia with reduced effector cell fratricide.
Gurney M, Stikvoort A, Nolan E, Kirkham-McCarthy L, Khoruzhenko S, Shivakumar R, Zweegman S, Van de Donk NWCJ, Mutis T, Szegezdi E, Sarkar S, O'Dwyer M. Gurney M, et al. Among authors: shivakumar r. Haematologica. 2022 Feb 1;107(2):437-445. doi: 10.3324/haematol.2020.271908. Haematologica. 2022. PMID: 33375774 Free PMC article.
Clinical scale electroloading of mature dendritic cells with melanoma whole tumor cell lysate is superior to conventional lysate co-incubation in triggering robust in vitro expansion of functional antigen-specific CTL.
Wolfraim LA, Takahara M, Viley AM, Shivakumar R, Nieda M, Maekawa R, Liu LN, Peshwa MV. Wolfraim LA, et al. Among authors: shivakumar r. Int Immunopharmacol. 2013 Mar;15(3):488-97. doi: 10.1016/j.intimp.2013.01.009. Epub 2013 Feb 8. Int Immunopharmacol. 2013. PMID: 23474736