3D quantification of autophagy activation and autophagosome-to-mitochondria recruitment in a Drosophila model of Parkinson's disease

Susana J Gutierrez-Luke; Amber N Juba; Giulia Bertolin; Lori M Buhlman

doi:10.1016/j.xpro.2021.100408

3D quantification of autophagy activation and autophagosome-to-mitochondria recruitment in a Drosophila model of Parkinson's disease

STAR Protoc. 2021 Mar 25;2(2):100408. doi: 10.1016/j.xpro.2021.100408. eCollection 2021 Jun 18.

Authors

Susana J Gutierrez-Luke¹, Amber N Juba², Giulia Bertolin³, Lori M Buhlman²

Affiliations

¹ Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308 USA.
² College of Graduate Studies, Midwestern University, Glendale, AZ 85308 USA.
³ Univ Rennes, CNRS, IGDR (Genetics and Development Institute of Rennes), UMR 6290, 35000 Rennes, France.

Abstract

Here, we describe a protocol for comprehensive quantification of autophagosome recruitment to mitochondria as an early step in mitophagy. Data collected using this protocol can be useful in the study of neurodegenerative disease, cancer, and metabolism-related disorders using models in which co-expression of mito-GFP and mCherry-Atg8a is feasible. This protocol has the advantage of assessment in an in vivo model organism (Drosophila melanogaster), where tissue-specific mitophagy can be investigated. For complete details on the use and execution of this protocol, please refer to (Cackovic et al., 2018).

Keywords: Cell Biology; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autophagosomes* / metabolism
Autophagosomes* / pathology
Autophagy / physiology*
Brain / cytology
Brain / metabolism
Brain / pathology
Disease Models, Animal
Drosophila / cytology
Drosophila / metabolism
Microscopy, Confocal / methods
Mitochondria* / metabolism
Mitochondria* / pathology
Mitophagy
Molecular Imaging / methods*
Parkinson Disease* / metabolism
Parkinson Disease* / pathology