Toxoplasmosis, caused by Toxoplasma gondii, is one of the most prevalent parasite illnesses in humans. Although primary infection in a pregnant woman is usually asymptomatic, it has the potential to cause significant harm to the fetus, including miscarriage. In this study, we investigate the usefulness of the PCR to confirm the etiology of the abortion. A prospective study in the Al-Diwaniyah maternity and pediatric teaching hospital in Iraq was conducted. The research comprised 94 aborted women. We have reported a new internal primer for the nested PCR protocol to detect toxoplasmosis. In the 94-aborted women, 30 samples (31.9 %) were positive by the nPCR using the G529 repeat gene and qPCR using B1 gene primers. We have shown that three women carry the parasite in their placentas, and at the same time, they do not carry antibodies in their blood. We recommend that women should be aware of the risk of toxoplasmosis and the importance of preventing measures. In addition, PCR should be done in the case of abortion to enhance sensitivity even if serology is negative.