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Massively parallel Cas13 screens reveal principles for guide RNA design.
Wessels HH, Méndez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Wessels HH, et al. Among authors: mendez mancilla a. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. Nat Biotechnol. 2020. PMID: 32518401 Free PMC article.
Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells.
Méndez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker J, Robb GB, Holden K, Sanjana NE. Méndez-Mancilla A, et al. Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2. Cell Chem Biol. 2022. PMID: 34343484 Free PMC article.
Low copy CRISPR-Cas13d mitigates collateral RNA cleavage.
Hart SK, Wessels HH, Méndez-Mancilla A, Müller S, Drabavicius G, Choi O, Sanjana NE. Hart SK, et al. Among authors: mendez mancilla a. bioRxiv [Preprint]. 2024 May 14:2024.05.13.594039. doi: 10.1101/2024.05.13.594039. bioRxiv. 2024. PMID: 38798586 Free PMC article. Preprint.
Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens.
Liscovitch-Brauer N, Montalbano A, Deng J, Méndez-Mancilla A, Wessels HH, Moss NG, Kung CY, Sookdeo A, Guo X, Geller E, Jaini S, Smibert P, Sanjana NE. Liscovitch-Brauer N, et al. Among authors: mendez mancilla a. Nat Biotechnol. 2021 Oct;39(10):1270-1277. doi: 10.1038/s41587-021-00902-x. Epub 2021 Apr 29. Nat Biotechnol. 2021. PMID: 33927415 Free PMC article.
Increased levels of adipose tissue-resident Th17 cells in obesity associated with miR-326.
Vega-Cárdenas M, Uresti-Rivera EE, Cortés-García JD, Briones-Espinoza M, Ruíz-Rodríguez VM, Reynaga-Hernández E, Mendez-Mancilla A, Portales-Pérez DP. Vega-Cárdenas M, et al. Among authors: mendez mancilla a. Immunol Lett. 2019 Jul;211:60-67. doi: 10.1016/j.imlet.2019.05.010. Epub 2019 May 25. Immunol Lett. 2019. PMID: 31136754
11 results