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The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods.
Kemp LE, Alphey MS, Bond CS, Ferguson MA, Hecht S, Bacher A, Eisenreich W, Rohdich F, Hunter WN. Kemp LE, et al. Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):45-52. doi: 10.1107/S0907444904025971. Epub 2004 Dec 17. Acta Crystallogr D Biol Crystallogr. 2005. PMID: 15608374
A double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate.
Sgraja T, Kemp LE, Ramsden N, Hunter WN. Sgraja T, et al. Among authors: kemp le. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt 7):625-9. doi: 10.1107/S1744309105018762. Epub 2005 Jun 30. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005. PMID: 16511114 Free PMC article.
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