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Structural and immunological characterization of O-acetylated GD2. Evidence that GD2 is an acceptor for ganglioside O-acetyltransferase in human melanoma cells.
Sjoberg ER, Manzi AE, Khoo KH, Dell A, Varki A. Sjoberg ER, et al. Among authors: varki a. J Biol Chem. 1992 Aug 15;267(23):16200-11. J Biol Chem. 1992. PMID: 1644805 Free article.
We have shown previously that Golgi-enriched vesicles from the human melanoma cell line Melur can transfer [3H]acetate from [acetyl-3H]acetyl-CoA to endogenous GD3 to form [acetyl-3H]O-acetyl-GD3 (Manzi, A. E., Sjoberg, E. R., Diaz, S., and Varki, A. (1990) J …
We have shown previously that Golgi-enriched vesicles from the human melanoma cell line Melur can transfer [3H]acetate from [acetyl-3H]acety …
O-acetylation and de-O-acetylation of sialic acids. O-acetylation of sialic acids in the rat liver Golgi apparatus involves an acetyl intermediate and essential histidine and lysine residues--a transmembrane reaction?
Higa HH, Butor C, Diaz S, Varki A. Higa HH, et al. Among authors: varki a. J Biol Chem. 1989 Nov 15;264(32):19427-34. J Biol Chem. 1989. PMID: 2509477 Free article.
Isolated intact rat liver Golgi vesicles utilize [acetyl-3H]coenzyme A to add 3H-O-acetyl esters to sialic acids of internally facing endogenous glycoproteins. ...On the other hand, entry of intact AcCoA into the lumen of the vesicles could not be demonstrated, and permeab …
Isolated intact rat liver Golgi vesicles utilize [acetyl-3H]coenzyme A to add 3H-O-acetyl esters to sialic acids of internally facing …
O-acetylation and de-O-acetylation of sialic acids. 7- and 9-o-acetylation of alpha 2,6-linked sialic acids on endogenous N-linked glycans in rat liver Golgi vesicles.
Diaz S, Higa HH, Hayes BK, Varki A. Diaz S, et al. Among authors: varki a. J Biol Chem. 1989 Nov 15;264(32):19416-26. J Biol Chem. 1989. PMID: 2808433 Free article.
The incorporated radioactivity occurred in acid-soluble and acid-insoluble components, and the acid-insoluble fraction included O-acetylated sialic acids (Varki, A., and Diaz, S. (1985) J. Biol. Chem. 260, 6600-6608). Nearly all of the protein-associated radioactivi …
The incorporated radioactivity occurred in acid-soluble and acid-insoluble components, and the acid-insoluble fraction included O-acetylated …
O-acetylation and de-O-acetylation of sialic acids. Purification, characterization, and properties of a glycosylated rat liver esterase specific for 9-O-acetylated sialic acids.
Higa HH, Manzi A, Varki A. Higa HH, et al. Among authors: varki a. J Biol Chem. 1989 Nov 15;264(32):19435-42. J Biol Chem. 1989. PMID: 2808434 Free article.
We have previously described the preparation and use of 9-O-[acetyl-3H]acetyl-N-acetylneuraminic acid to identify sialic acid O-acetylesterases in tissues and cells (Higa, H. H., Diaz, S., and Varki, A. (1987) Biochem. Biophys. Res. Commun. 144, 1099-1108). All tiss …
We have previously described the preparation and use of 9-O-[acetyl-3H]acetyl-N-acetylneuraminic acid to identify sialic acid O-acetylestera …
Sulfated N-linked oligosaccharides in mammalian cells. I. Complex-type chains with sialic acids and O-sulfate esters.
Roux L, Holojda S, Sundblad G, Freeze HH, Varki A. Roux L, et al. Among authors: varki a. J Biol Chem. 1988 Jun 25;263(18):8879-89. J Biol Chem. 1988. PMID: 3379050 Free article.
H., and Varki, A. (1986) Biochem. Biophys. Res. Commun. 140, 967-973). Here we report improved methods to metabolically label cell lines with 35SO4 and to release sulfated N-linked oligosaccharides with peptide:N-glycosidase F as well as the partial structure of som …
H., and Varki, A. (1986) Biochem. Biophys. Res. Commun. 140, 967-973). Here we report improved methods to metabolically label …
559 results