A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera).
Koiwa H, Shade RE, Zhu-Salzman K, D'Urzo MP, Murdock LL, Bressan RA, Hasegawa PM.
Koiwa H, et al.
FEBS Lett. 2000 Apr 7;471(1):67-70. doi: 10.1016/s0014-5793(00)01368-5.
FEBS Lett. 2000.
PMID: 10760514
Free article.
WCR digestive proteolytic activity was separated by mildly denaturing SDS-PAGE into two fractions and in-gel assays confirmed that the proteinase activities of each were largely scN-sensitive. Since binding affinity to the target proteinase [Koiwa, H. et al. (1998) …
WCR digestive proteolytic activity was separated by mildly denaturing SDS-PAGE into two fractions and in-gel assays confirmed that the prote …