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Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris.
Yurugi-Kobayashi T, Asada H, Shiroishi M, Shimamura T, Funamoto S, Katsuta N, Ito K, Sugawara T, Tokuda N, Tsujimoto H, Murata T, Nomura N, Haga K, Haga T, Iwata S, Kobayashi T. Yurugi-Kobayashi T, et al. Biochem Biophys Res Commun. 2009 Mar 6;380(2):271-6. doi: 10.1016/j.bbrc.2009.01.053. Epub 2009 Jan 22. Biochem Biophys Res Commun. 2009. PMID: 19167344
G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody.
Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T. Hino T, et al. Among authors: yurugi kobayashi t. Nature. 2012 Jan 29;482(7384):237-40. doi: 10.1038/nature10750. Nature. 2012. PMID: 22286059 Free PMC article.
Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: yurugi kobayashi t. Biochim Biophys Acta. 2010 Sep;1800(9):986-92. doi: 10.1016/j.bbagen.2010.06.003. Epub 2010 Jun 11. Biochim Biophys Acta. 2010. PMID: 20542090 Free article.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: yurugi kobayashi t. Biotechnol Lett. 2011 Jan;33(1):103-7. doi: 10.1007/s10529-010-0399-1. Epub 2010 Oct 9. Biotechnol Lett. 2011. PMID: 20936326 Free article.
Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium.
Tokuda N, Igarashi K, Shimamura T, Yurugi-Kobayashi T, Shiroishi M, Ito K, Sugawara T, Asada H, Murata T, Nomura N, Iwata S, Kobayashi T. Tokuda N, et al. Among authors: yurugi kobayashi t. Protein Expr Purif. 2011 Sep;79(1):81-7. doi: 10.1016/j.pep.2011.04.006. Epub 2011 Apr 15. Protein Expr Purif. 2011. PMID: 21515379 Free article.
Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.
Ito K, Sugawara T, Shiroishi M, Tokuda N, Kurokawa A, Misaka T, Makyio H, Yurugi-Kobayashi T, Shimamura T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T. Ito K, et al. Among authors: yurugi kobayashi t. Biochem Biophys Res Commun. 2008 Jul 11;371(4):841-5. doi: 10.1016/j.bbrc.2008.04.182. Epub 2008 May 12. Biochem Biophys Res Commun. 2008. PMID: 18474222
19 results