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CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.
Westra ER, van Erp PB, Künne T, Wong SP, Staals RH, Seegers CL, Bollen S, Jore MM, Semenova E, Severinov K, de Vos WM, Dame RT, de Vries R, Brouns SJ, van der Oost J. Westra ER, et al. Among authors: staals rh. Mol Cell. 2012 Jun 8;46(5):595-605. doi: 10.1016/j.molcel.2012.03.018. Epub 2012 Apr 19. Mol Cell. 2012. PMID: 22521689 Free PMC article.
Degenerate target sites mediate rapid primed CRISPR adaptation.
Fineran PC, Gerritzen MJ, Suárez-Diez M, Künne T, Boekhorst J, van Hijum SA, Staals RH, Brouns SJ. Fineran PC, et al. Among authors: staals rh. Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1629-38. doi: 10.1073/pnas.1400071111. Epub 2014 Apr 7. Proc Natl Acad Sci U S A. 2014. PMID: 24711427 Free PMC article.
RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.
Staals RH, Zhu Y, Taylor DW, Kornfeld JE, Sharma K, Barendregt A, Koehorst JJ, Vlot M, Neupane N, Varossieau K, Sakamoto K, Suzuki T, Dohmae N, Yokoyama S, Schaap PJ, Urlaub H, Heck AJ, Nogales E, Doudna JA, Shinkai A, van der Oost J. Staals RH, et al. Mol Cell. 2014 Nov 20;56(4):518-30. doi: 10.1016/j.molcel.2014.10.005. Epub 2014 Nov 6. Mol Cell. 2014. PMID: 25457165 Free PMC article.
The Cpf1 CRISPR-Cas protein expands genome-editing tools.
Fagerlund RD, Staals RH, Fineran PC. Fagerlund RD, et al. Among authors: staals rh. Genome Biol. 2015 Nov 17;16:251. doi: 10.1186/s13059-015-0824-9. Genome Biol. 2015. PMID: 26578176 Free PMC article.
47 results