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Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice.
Marugán-Hernández V, Ortega-Mora LM, Aguado-Martínez A, Jiménez-Ruíz E, Alvarez-García G. Marugán-Hernández V, et al. Vaccine. 2011 Oct 13;29(44):7867-74. doi: 10.1016/j.vaccine.2011.07.091. Epub 2011 Aug 2. Vaccine. 2011. PMID: 21816191
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