Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR

K Becker; D Badehorn; B Keller; M Schulte; K H Böhm; G Peters; W Fegeler

doi:10.1128/JCM.39.9.3356-3359.2001

Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR

J Clin Microbiol. 2001 Sep;39(9):3356-9. doi: 10.1128/JCM.39.9.3356-3359.2001.

Authors

K Becker¹, D Badehorn, B Keller, M Schulte, K H Böhm, G Peters, W Fegeler

Affiliation

¹ Institute of Medical Microbiology, University of Münster, D-48149 Münster, Germany. kbecker@uni-muenster.de

Abstract

A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.

Publication types

Evaluation Study

MeSH terms

Candida / classification*
Candida / genetics*
DNA Primers
DNA, Fungal / genetics
DNA, Fungal / isolation & purification*
Humans
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Species Specificity

Substances

DNA Primers
DNA, Fungal

Associated data

GENBANK/AJ289782