Abstract
A moderately halophilic, aerobic, motile, Gram-negative, rod-shaped bacterium (strain LV4(T)) was isolated from saline soil around the lake Laguna Verde in the Bolivian Andes. The organism is a heterotroph, able to utilize various carbohydrates as a carbon source. It showed tryptophan deaminase, oxidase and catalase activity, but was unable to produce indole or H(2)S; nitrate was not reduced. The G+C content of the genomic DNA was 56.1 mol%. The pH range for growth was 5-10, temperature range was 0-45 degrees C and the range of NaCl concentrations was 0-25 % (w/v). On the basis of 16S rRNA gene sequence analysis, strain LV4(T) was found to be closely related to Chromohalobacter canadensis DSM 6769(T) and Pseudomonas beijerinckii DSM 7218(T); however, its DNA-DNA relatedness with these type strains was low. Strain LV4(T) resembled other Chromohalobacter species with respect to various physiological, biochemical and nutritional characteristics but also exhibited differences. Thus, a novel species, Chromohalobacter sarecensis sp. nov., is proposed, with LV4(T) (=CCUG 47987(T)=ATCC BAA-761(T)) as the type strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Oxidoreductases / analysis
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Bacterial Typing Techniques
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Base Composition
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Bolivia
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Carbohydrate Metabolism
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Catalase / analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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Genes, rRNA
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Gentian Violet
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Halomonadaceae / classification*
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Halomonadaceae / cytology
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Halomonadaceae / genetics
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Halomonadaceae / isolation & purification*
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Halomonadaceae / metabolism
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Halomonadaceae / physiology
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Hydrogen Sulfide / metabolism
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Hydrogen-Ion Concentration
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Indoles / metabolism
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L-Amino Acid Oxidase
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Molecular Sequence Data
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Movement
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Nucleic Acid Hybridization
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Oxidoreductases / analysis
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Phenazines
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Phylogeny
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Pseudomonas / genetics
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Saline Solution, Hypertonic
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Sequence Analysis, DNA
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Soil Microbiology*
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Temperature
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Gram's stain
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Indoles
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Phenazines
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Saline Solution, Hypertonic
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indole
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Oxidoreductases
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Catalase
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Amino Acid Oxidoreductases
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L-Amino Acid Oxidase
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Gentian Violet
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Hydrogen Sulfide