Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties

Vaccine. 1991 Dec;9(12):901-6. doi: 10.1016/0264-410x(91)90011-t.

Abstract

Acellular whooping cough vaccines are based on pertussis toxoid but their effectiveness may be increased by the addition of other Bordetella pertussis antigens. We expressed the immunogenic outer membrane protein pertactin (P69) from B. pertussis to high levels in multi-copy transformants of the industrial yeast Pichia pastoris. In high-density fermentations, engineered P. pastoris yielded greater than 3 g of the protein per litre of culture. Purified recombinant pertactin was able to stimulate the incomplete protection afforded by toxoid to the level of the whole-cell vaccine, as shown by the Kendrick test, supporting its inclusion in future acellular vaccines.

MeSH terms

  • Bacterial Outer Membrane Proteins / biosynthesis
  • Bacterial Outer Membrane Proteins / immunology*
  • Base Sequence
  • Blotting, Western
  • Bordetella pertussis / immunology*
  • Fermentation
  • Gene Expression
  • Genetic Vectors / genetics
  • Molecular Sequence Data
  • Pertussis Vaccine / administration & dosage*
  • Pichia / genetics
  • Pichia / metabolism
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / immunology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Transformation, Genetic
  • Virulence Factors, Bordetella*
  • Whooping Cough / prevention & control*

Substances

  • Bacterial Outer Membrane Proteins
  • Pertussis Vaccine
  • Recombinant Proteins
  • Virulence Factors, Bordetella
  • pertactin