Purification, molecular cloning, and enzymatic properties of a family 12 endoglucanase (EG-II) from fomitopsis palustris: role of EG-II in larch holocellulose hydrolysis

Appl Environ Microbiol. 2008 Sep;74(18):5857-61. doi: 10.1128/AEM.00435-08. Epub 2008 Jul 25.

Abstract

A family 12 endoglucanase with a molecular mass of 23,926 Da (EG-II) from the brown-rot basidiomycete Fomitopsis palustris was purified and characterized. One of the roles of EG-II in wood degradation is thought to be to loosen the polysaccharide network in cell walls by disentangling hemicelluloses that are associated with cellulose.

MeSH terms

  • Cellulase / genetics*
  • Cellulose / metabolism*
  • Cloning, Molecular
  • Genes, Fungal
  • Hydrolysis
  • Larix / microbiology
  • Molecular Sequence Data
  • Polyporales / enzymology*
  • Polyporales / genetics*
  • Polyporales / metabolism
  • RNA, Fungal / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Substrate Specificity
  • Wood / metabolism

Substances

  • RNA, Fungal
  • Cellulose
  • endoglucanase 2
  • Cellulase

Associated data

  • GENBANK/AB299016