Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei

Gene. 1989 Apr 15;77(1):11-9. doi: 10.1016/0378-1119(89)90354-5.

Abstract

R.MwoI, a type-II restriction enzyme with the new specificity 5'-GCN7GC-3', was found in extracts of the thermophilic archaebacterium, Methanobacterium wolfei. R.MwoI cleaves duplex DNA producing fragments with 3-nt, 3'-terminal extensions, thus: GCN5/N2GC. The genes coding for the MwoI restriction and modification enzymes were cloned into Escherichia coli on the plasmid vector pBR322. The clones synthesize a low level of R.MwoI endonuclease. The plasmids display incomplete MwoI-specific modification, suggesting that the clones synthesize a low level of the M.MwoI methyltransferase, too.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cloning, Molecular*
  • DNA, Bacterial / isolation & purification
  • Deoxyribonucleases, Type II Site-Specific / genetics*
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Escherichia coli / genetics
  • Euryarchaeota / enzymology
  • Euryarchaeota / genetics*
  • Euryarchaeota / growth & development
  • Genes, Bacterial*
  • Plasmids
  • Substrate Specificity

Substances

  • DNA, Bacterial
  • endodeoxyribonuclease MwoI
  • Deoxyribonucleases, Type II Site-Specific