Molecular cloning of a gene sequence regulated by nerve growth factor

Science. 1985 Jul 26;229(4711):393-5. doi: 10.1126/science.3839317.

Abstract

Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in a cultured target cell line of pheochromocytoma cells (PC12 cells) 5 hours after the addition of NGF. The induced messenger RNA encodes a 90,000-dalton polypeptide that may represent one of the primary events in NGF-induced differentiation of neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Adrenal Gland Neoplasms / genetics
  • Animals
  • Base Sequence
  • Cell Line
  • Chickens
  • Cloning, Molecular*
  • DNA / genetics
  • Gene Expression Regulation
  • Genes*
  • Nerve Growth Factors / physiology*
  • Nucleic Acid Hybridization
  • Pheochromocytoma / genetics
  • RNA, Messenger / genetics
  • Rabbits
  • Rats

Substances

  • Actins
  • Nerve Growth Factors
  • RNA, Messenger
  • DNA