Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus

C Ash; F G Priest; M D Collins

doi:10.1007/BF00873085

Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus

Antonie Van Leeuwenhoek. 1993;64(3-4):253-60. doi: 10.1007/BF00873085.

Authors

C Ash¹, F G Priest, M D Collins

Affiliation

¹ Department of Microbiology, Agricultural and Food Research Council, Institute of Food Research, Reading, UK.

PMID: 8085788
DOI: 10.1007/BF00873085

Abstract

Comparative 16S rRNA sequence analysis has demonstrated that the genus Bacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprising Bacillus polymyxa and close relatives is phylogenetically so removed from Bacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genus Paenibacillus. The genus Paenibacillus can be readily distinguished from other Bacillus groups using a battery of phenotypic characters and a highly specific gene probe based on 16S rRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / classification*
Bacillus / genetics*
Base Sequence
Classification / methods
DNA Primers
Molecular Sequence Data
Phylogeny*
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics*

Substances

DNA Primers
RNA, Ribosomal, 16S

Associated data

GENBANK/M77489