Identification, sequence, and expression of caveolin-2 defines a caveolin gene family

Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):131-5. doi: 10.1073/pnas.93.1.131.

Abstract

Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Here, an approximately 20-kDa caveolin-related protein, caveolin-2, was identified through microsequencing of adipocyte-derived caveolin-enriched membranes; caveolin was retermed caveolin-1. Caveolins 1 and 2 are similar in most respects. mRNAs for both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during adipocyte differentiation. Caveolin-2 colocalizes with caveolin-1, indicating that caveolin-2 also localizes to caveolae. However, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Adipocytes / chemistry*
  • Amino Acid Sequence
  • Animals
  • Caveolin 1
  • Caveolins*
  • Chickens
  • Fluorescent Antibody Technique, Indirect
  • GTP-Binding Proteins / metabolism
  • Gene Expression
  • Humans
  • Membrane Proteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • CAV1 protein, human
  • Cav1 protein, mouse
  • Caveolin 1
  • Caveolins
  • Membrane Proteins
  • RNA, Messenger
  • GTP-Binding Proteins

Associated data

  • GENBANK/AF035752
  • GENBANK/AJ010316