Lactobacillus bulgaricus asparagine synthetase and asparaginyl-tRNA synthetase: coregulation by transcription antitermination?

S I Kim; J E Germond; D Pridmore; D Söll

doi:10.1128/jb.178.8.2459-2461.1996

Lactobacillus bulgaricus asparagine synthetase and asparaginyl-tRNA synthetase: coregulation by transcription antitermination?

J Bacteriol. 1996 Apr;178(8):2459-61. doi: 10.1128/jb.178.8.2459-2461.1996.

Authors

S I Kim¹, J E Germond, D Pridmore, D Söll

Affiliation

¹ Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA.

Abstract

Genes encoding the ammonia-dependent asparagine synthetase (asnA) and asparaginyl-tRNA synthetase (asnS) have been cloned from Lactobacillus bulgaricus ATCC 11842. The nucleotide sequence suggests that asnA and asnS are organized as one operon and regulated by the tRNA-directed transcription antitermination mechanism (T. M. Henkin, Mol. Microbiol. 13:381-387, 1994).

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Amino Acyl-tRNA Synthetases / biosynthesis
Amino Acyl-tRNA Synthetases / genetics*
Aspartate-Ammonia Ligase / biosynthesis
Aspartate-Ammonia Ligase / genetics*
Aspartate-tRNA Ligase*
Base Sequence
Gene Expression Regulation, Bacterial*
Genes, Bacterial
Genetic Complementation Test
Lactobacillus / enzymology
Lactobacillus / genetics*
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Transfer, Amino Acyl*
Sequence Homology, Amino Acid
Terminator Regions, Genetic*
Transcription, Genetic*

Substances

RNA, Transfer, Amino Acyl
Amino Acyl-tRNA Synthetases
Aspartate-tRNA Ligase
asparaginyl-tRNA synthetase
Aspartate-Ammonia Ligase

Associated data

GENBANK/X89438
GENBANK/X89439