Cervera J - Search Results

1

Carbamate kinase from Enterococcus faecalis and Enterococcus faecium--cloning of the genes, studies on the enzyme expressed in Escherichia coli, and sequence similarity with N-acetyl-L-glutamate kinase.

Marina A, Uriarte M, Barcelona B, Fresquet V, Cervera J, Rubio V. Marina A, et al. Among authors: cervera j. Eur J Biochem. 1998 Apr 1;253(1):280-91. doi: 10.1046/j.1432-1327.1998.2530280.x. Eur J Biochem. 1998. PMID: 9578487 Free article.

2

The carbamoyl-phosphate synthase family and carbamate kinase: structure-function studies.

Rubio V, Cervera J. Rubio V, et al. Among authors: cervera j. Biochem Soc Trans. 1995 Nov;23(4):879-83. doi: 10.1042/bst0230879. Biochem Soc Trans. 1995. PMID: 8654858 Review. No abstract available.

3

Photoaffinity labeling with the activator IMP and site-directed mutagenesis of histidine 995 of carbamoyl phosphate synthetase from Escherichia coli demonstrate that the binding site for IMP overlaps with that for the inhibitor UMP.

Bueso J, Cervera J, Fresquet V, Marina A, Lusty CJ, Rubio V. Bueso J, et al. Among authors: cervera j. Biochemistry. 1999 Mar 30;38(13):3910-7. doi: 10.1021/bi982871f. Biochemistry. 1999. PMID: 10194302

However, the mutation selectively induced changes in the activation by IMP and the inhibition by UMP, and it abolished the photolabeling of the enzyme by IMP without affecting the photolabeling by the inhibitor UMP. Since UMP is cross-linked to Lys993 [Cervera, J., …

However, the mutation selectively induced changes in the activation by IMP and the inhibition by UMP, and it abolished the photolabeling of …

4

Carbamate kinase can replace in vivo carbamoyl phosphate synthetase. Implications for the evolution of carbamoyl phosphate biosynthesis.

Alcántara C, Cervera J, Rubio V. Alcántara C, et al. Among authors: cervera j. FEBS Lett. 2000 Nov 10;484(3):261-4. doi: 10.1016/s0014-5793(00)02168-2. FEBS Lett. 2000. PMID: 11078889 Free article.

5

Mechanism of oligomerization of Escherichia coli carbamoyl phosphate synthetase and modulation by the allosteric effectors. A site-directed mutagenesis study.

Mora P, Rubio V, Cervera J. Mora P, et al. Among authors: cervera j. FEBS Lett. 2002 Jan 30;511(1-3):6-10. doi: 10.1016/s0014-5793(01)03246-x. FEBS Lett. 2002. PMID: 11821039 Free article.

6

Mechanism of allosteric modulation of Escherichia coli carbamoyl phosphate synthetase probed by site-directed mutagenesis of ornithine site residues.

Rochera L, Fresquet V, Rubio V, Cervera J. Rochera L, et al. Among authors: cervera j. FEBS Lett. 2002 Mar 13;514(2-3):323-8. doi: 10.1016/s0014-5793(02)02392-x. FEBS Lett. 2002. PMID: 11943174 Free article.

7

Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.

Pérez-Arellano I, Gil-Ortiz F, Cervera J, Rubio V. Pérez-Arellano I, et al. Among authors: cervera j. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2091-4. doi: 10.1107/S0907444904023972. Epub 2004 Oct 20. Acta Crystallogr D Biol Crystallogr. 2004. PMID: 15502337

8

Understanding carbamoyl phosphate synthetase deficiency: impact of clinical mutations on enzyme functionality.

Yefimenko I, Fresquet V, Marco-Marín C, Rubio V, Cervera J. Yefimenko I, et al. Among authors: cervera j. J Mol Biol. 2005 May 27;349(1):127-41. doi: 10.1016/j.jmb.2005.03.078. Epub 2005 Apr 12. J Mol Biol. 2005. PMID: 15876373

9

Dissection of Escherichia coli glutamate 5-kinase: functional impact of the deletion of the PUA domain.

Pérez-Arellano I, Rubio V, Cervera J. Pérez-Arellano I, et al. Among authors: cervera j. FEBS Lett. 2005 Dec 19;579(30):6903-8. doi: 10.1016/j.febslet.2005.11.037. Epub 2005 Dec 1. FEBS Lett. 2005. PMID: 16337196 Free article.

10

Mapping active site residues in glutamate-5-kinase. The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites.

Pérez-Arellano I, Rubio V, Cervera J. Pérez-Arellano I, et al. Among authors: cervera j. FEBS Lett. 2006 Nov 13;580(26):6247-53. doi: 10.1016/j.febslet.2006.10.031. Epub 2006 Oct 20. FEBS Lett. 2006. PMID: 17069808 Free article.

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