Simultaneous analysis of retinol, six carotenoids, two tocopherols, and coenzyme Q10 from human plasma by HPLC

Lysiane Boulet; Brigitte Alex; Nadège Clavey; Julie Martinez; Véronique Ducros

doi:10.1016/j.jchromb.2020.122158

Simultaneous analysis of retinol, six carotenoids, two tocopherols, and coenzyme Q10 from human plasma by HPLC

J Chromatogr B Analyt Technol Biomed Life Sci. 2020 May 29:1151:122158. doi: 10.1016/j.jchromb.2020.122158. Online ahead of print.

Authors

Lysiane Boulet¹, Brigitte Alex¹, Nadège Clavey¹, Julie Martinez¹, Véronique Ducros²

Affiliations

¹ Nutrition Unit, Biochemistry Laboratory, University Hospital Grenoble-Alpes, 38043 Grenoble cedex 9, France.
² Nutrition Unit, Biochemistry Laboratory, University Hospital Grenoble-Alpes, 38043 Grenoble cedex 9, France. Electronic address: v-ducros@orange.fr.

PMID: 32531701
DOI: 10.1016/j.jchromb.2020.122158

Abstract

Lipophilic antioxidant determination is of relevance in health and diseases. Several HPLC methods exists but rare are those including coenzyme Q10 with carotenoids, retinol and tocopherols. Here a single-step extraction was proposed for the detection of retinol, α and γ-tocopherols, lutein, zeaxanthin, trans-ß-carotene, α-carotene, ß-cryptoxanthin and lycopene as well as coenzyme Q10. A single HPLC column was used and UV-vis diode array detection was performed. Echinenone, alpha-tocopherol nicotinate and coenzyme Q4 were employed as internal standards. Intra-assay and inter-assay precision were respectively 1.4-7.9% and 2.2-15.8%. Accuracy was validated using SRM 968e. LOD (limit of detection) and LOQ (limit of quantification) obtained were sufficient for nutritional epidemiological study and routine clinical application.