Low molecular weight poly((D,L)-lactide- co-caprolactone) liquid inks for diluent-free DLP printing of cell culture platforms

Biomater Sci. 2023 Jul 25;11(15):5163-5176. doi: 10.1039/d3bm00581j.

Abstract

Digital light processing (DLP) printing offers the possibility of fabricating complex objects in a fast and reproducible manner. A main requirement for DLP printing is the use of inks with low viscosities that can flow under the printing platform in a short period of time. Its exploitation in tissue engineering applications has been centered on the use of hydrogel forming materials diluted in aqueous solutions or the use of polyesters in combination with diluents and heating platforms that aid in the reduction of their viscosity. The use of diluents, however, modifies the mechanical properties and reduces the shape fidelity of the printed objects and, the use of heating platforms results in vats with heterogeneous temperatures and ink viscosities. Here, we report on the synthesis of a library of methacrylated low molecular weight (<3000 g mol-1) homopolymers ((P(D,L)LA and PCL) and copolymers (P((D,L)LA-co-CL)) of 2- and 3-arms based on (D,L)-lactide and ε-caprolactone. The resulting inks possessed low viscosity that made them printable in the absence of diluents and heating elements. DLP printing of cubical and cylindrical patterns resulted in objects with a higher shape fidelity than their counterparts fabricated using diluents and with printed features on the order of 300 μm. The printed materials were biocompatible and supported the growth of human mesenchymal stem cells (hMSCs). Moreover, the variations in the composition resulted in polymers that enabled the attachment of hMSCs to different extents, leading to the formation of well-adhered cell monolayers or loosely adhered cell aggregates.

MeSH terms

  • Biocompatible Materials*
  • Cell Culture Techniques
  • Humans
  • Ink*
  • Molecular Weight
  • Polyesters
  • Polymers
  • Printing, Three-Dimensional
  • Tissue Engineering

Substances

  • Biocompatible Materials
  • caprolactone
  • dilactide
  • Polymers
  • Polyesters