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Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG. Aceti DJ, et al. J Struct Funct Genomics. 2015 Jun;16(2):67-80. doi: 10.1007/s10969-015-9198-1. Epub 2015 Apr 9. J Struct Funct Genomics. 2015. PMID: 25854603 Free PMC article.
Wheat germ cell-free expression system for protein production.
Vinarov DA, Newman CLL, Tyler EM, Markley JL, Shahan MN. Vinarov DA, et al. Curr Protoc Protein Sci. 2006 Jun;Chapter 5:5.18.1-5.18.18. doi: 10.1002/0471140864.ps0518s44. Curr Protoc Protein Sci. 2006. PMID: 18429309
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