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Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials.
Goud GN, Bottazzi ME, Zhan B, Mendez S, Deumic V, Plieskatt J, Liu S, Wang Y, Bueno L, Fujiwara R, Samuel A, Ahn SY, Solanki M, Asojo OA, Wang J, Bethony JM, Loukas A, Roy M, Hotez PJ. Goud GN, et al. Vaccine. 2005 Sep 15;23(39):4754-64. doi: 10.1016/j.vaccine.2005.04.040. Vaccine. 2005. PMID: 16054275
Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus.
Zhan B, Perally S, Brophy PM, Xue J, Goud G, Liu S, Deumic V, de Oliveira LM, Bethony J, Bottazzi ME, Jiang D, Gillespie P, Xiao SH, Gupta R, Loukas A, Ranjit N, Lustigman S, Oksov Y, Hotez P. Zhan B, et al. Infect Immun. 2010 Apr;78(4):1552-63. doi: 10.1128/IAI.00848-09. Epub 2010 Feb 9. Infect Immun. 2010. PMID: 20145100 Free PMC article.
Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus.
Asojo OA, Goud GN, Zhan B, Ordonez K, Sedlacek M, Homma K, Deumic V, Gupta R, Brelsford J, Price MK, Ngamelue MN, Hotez PJ. Asojo OA, et al. Among authors: goud gn. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):172-6. doi: 10.1107/S1744309109051616. Epub 2010 Jan 27. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010. PMID: 20124715 Free PMC article.
Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative.
Hotez PJ, Zhan B, Bethony JM, Loukas A, Williamson A, Goud GN, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao S, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Shepherd HR, Brandt W, Russell PK. Hotez PJ, et al. Among authors: goud gn. Int J Parasitol. 2003 Sep 30;33(11):1245-58. doi: 10.1016/s0020-7519(03)00158-9. Int J Parasitol. 2003. PMID: 13678639 Review.
Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen.
Goud GN, Deumic V, Gupta R, Brelsford J, Zhan B, Gillespie P, Plieskatt JL, Tsao EI, Hotez PJ, Bottazzi ME. Goud GN, et al. Protein Expr Purif. 2012 Jun;83(2):145-51. doi: 10.1016/j.pep.2012.03.013. Epub 2012 Apr 4. Protein Expr Purif. 2012. PMID: 22503665
Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum.
Zhan B, Liu S, Perally S, Xue J, Fujiwara R, Brophy P, Xiao S, Liu Y, Feng J, Williamson A, Wang Y, Bueno LL, Mendez S, Goud G, Bethony JM, Hawdon JM, Loukas A, Jones K, Hotez PJ. Zhan B, et al. Infect Immun. 2005 Oct;73(10):6903-11. doi: 10.1128/IAI.73.10.6903-6911.2005. Infect Immun. 2005. PMID: 16177370 Free PMC article.
21 results