A conserved klo-1-mpk-1 pathway regulates autophagy and modulates longevity in Caenorhabditis elegans

Biochem Biophys Res Commun. 2021 Jul 12:562:36-42. doi: 10.1016/j.bbrc.2021.05.042. Epub 2021 May 23.

Abstract

There are six different longevity models in Caenorhabditis elegans. Previous studies have identified several convergence points, such as hlh-30, daf-16, and klf-3, required for lifespan extension in these longevity models. However, it is not clear whether there other such convergence points. In this study, based on analysis of transcriptome data, we found that the expression of klo-1/klotho was elevated in several longevity models. klo-1 was required for lifespan extension in the glp-1(e2141) and isp-1(qm150) mutants. klo-1 extended the lifespan of glp-1(e2141) and isp-1(qm150) worms by activating extracellular-signal-regulated kinase (ERK). In addition, klo-1 and mpk-1 (the homologous gene encoding ERK) regulated autophagy in glp-1(e2141) mutants, suggesting that klo-1 regulates lifespan by activating autophagy.

Keywords: Autophagy; Klo-1; Longevity; Mpk-1; RNA sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy*
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Cellulases / genetics
  • Cellulases / metabolism*
  • Longevity*
  • MAP Kinase Signaling System
  • Mitogen-Activated Protein Kinase 1 / metabolism*
  • Mutation / genetics

Substances

  • Caenorhabditis elegans Proteins
  • Mitogen-Activated Protein Kinase 1
  • mpk-1 protein, C elegans
  • Cellulases
  • Klo-1 protein, C elegans