Urine metabolomics of rats with chronic atrophic gastritis

PLoS One. 2020 Nov 11;15(11):e0236203. doi: 10.1371/journal.pone.0236203. eCollection 2020.

Abstract

Background/aim: To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG).

Materials and methods: Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method.

Results: Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism.

Conclusion: By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / urine*
  • Disease Models, Animal*
  • Gastritis, Atrophic / pathology*
  • Gastritis, Atrophic / urine*
  • Male
  • Metabolic Networks and Pathways*
  • Metabolomics / methods*
  • Rats
  • Rats, Wistar

Substances

  • Biomarkers

Grants and funding

The grant of the Preliminary Mechanism and Efficacy Evaluation by the excellent scientific research and innovation teams at Shandong University of Traditional Chinese Medicine in the treatment of major diseases No. 220316 and Shandong province key research and development plan 2016CYJS08A01-6“Establishment of Clinical Diagnosis and Treatment Standard System of Precise Prescription for the Prevention and Treatment of Gastric Precancerous Lesions by Jingfang Pinellia Xiexin Decoction.