DNA binding specificity of MunI restriction endonuclease is controlled by pH and calcium ions: involvement of active site carboxylate residues.
Lagunavicius A, Grazulis S, Balciunaite E, Vainius D, Siksnys V.
Lagunavicius A, et al.
Biochemistry. 1997 Sep 16;36(37):11093-9. doi: 10.1021/bi963126a.
Biochemistry. 1997.
PMID: 9287152
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that at pH 8.3 in the absence of Mg2+, MunI restriction endonuclease exhibits little DNA binding specificity, as compared with the D83A and E98A m …
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that a …