Genome editing in plants: Advancing crop transformation and overview of tools

Plant Physiol Biochem. 2018 Oct:131:12-21. doi: 10.1016/j.plaphy.2018.05.009. Epub 2018 May 7.

Abstract

Genome manipulation technology is one of emerging field which brings real revolution in genetic engineering and biotechnology. Targeted editing of genomes pave path to address a wide range of goals not only to improve quality and productivity of crops but also permit to investigate the fundamental roots of biological systems. These goals includes creation of plants with valued compositional properties and with characters that confer resistance to numerous biotic and abiotic stresses. Numerous novel genome editing systems have been introduced during the past few years; these comprise zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). Genome editing technique is consistent for improving average yield to achieve the growing demands of the world's existing food famine and to launch a feasible and environmentally safe agriculture scheme, to more specific, productive, cost-effective and eco-friendly. These exciting novel methods, concisely reviewed herein, have verified themselves as efficient and reliable tools for the genetic improvement of plants.

Keywords: Crop improvement; Genetic innovation; Genome editing; Transcription.

Publication types

  • Review

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Crops, Agricultural / genetics*
  • Gene Editing / methods*
  • Genome, Plant / genetics
  • Mutagenesis, Site-Directed / methods
  • Plants / genetics*
  • Transcription Activator-Like Effector Nucleases / genetics
  • Zinc Finger Nucleases / genetics

Substances

  • Transcription Activator-Like Effector Nucleases
  • Zinc Finger Nucleases