Isolation of a gene encoding a putative polyamine transporter from Candida albicans, GPT1

Yeast. 2001 Apr;18(6):555-61. doi: 10.1002/yea.697.

Abstract

A gene encoding a transport protein from the pathogenic yeast, Candida albicans, has been isolated during a complementation experiment utilizing an ornithine decarboxylase-negative (spe1 Delta) strain of Saccharomyces cerevisiae. This gene restores gamma-aminobutyric acid (GABA) transport to a GABA transport-negative mutant of S. cerevisiae and encodes a protein which putatively allows transport of one or more of the polyamines. We have assigned the name GPT1 (GABA/polyamine transporter) to this gene.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida albicans / enzymology
  • Candida albicans / genetics*
  • Candida albicans / growth & development
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Fungal Proteins*
  • GABA Plasma Membrane Transport Proteins
  • Gene Library
  • Genes, Fungal*
  • Genetic Complementation Test
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Organic Anion Transporters*
  • Ornithine Decarboxylase / genetics
  • Ornithine Decarboxylase / metabolism
  • Polyamines / metabolism
  • Saccharomyces cerevisiae Proteins
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • gamma-Aminobutyric Acid / physiology

Substances

  • Carrier Proteins
  • Fungal Proteins
  • GABA Plasma Membrane Transport Proteins
  • GPT1 protein, Candida albicans
  • Membrane Proteins
  • Membrane Transport Proteins
  • Organic Anion Transporters
  • Polyamines
  • Saccharomyces cerevisiae Proteins
  • UGA4 protein, S cerevisiae
  • gamma-Aminobutyric Acid
  • Ornithine Decarboxylase

Associated data

  • GENBANK/AF080132