Abstract
The utilization of maltose by Clostridium acetobutylicum ATCC 824 was investigated. Glucose was used preferentially to maltose, when both substrates were present in the medium. Maltose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on maltose, but not glucose or sucrose, as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that the specificity for maltose is contained entirely within the membrane in this organism. A putative gene system for the maltose PTS was identified (from the C. acetobutylicum ATCC 824 genome sequence), encoding an enzyme II(Mal) and a maltose 6-phosphate hydrolase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Biological Transport
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Cell Fractionation
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Cell-Free System
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Clostridium / genetics
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Clostridium / metabolism*
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Genes, Bacterial
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Glucose / metabolism
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Maltose / metabolism*
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Molecular Sequence Data
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
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Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
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Phosphorylation
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Sequence Homology, Amino Acid
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Sucrose / metabolism
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alpha-Glucosidases / genetics
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alpha-Glucosidases / metabolism
Substances
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Bacterial Proteins
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Sucrose
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Maltose
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-maltose phosphotransferase
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maltose-6'-phosphate glucosidase
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alpha-Glucosidases
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Glucose