Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes

Takuya Yamanaka; Takahiro Imai; Rena Satoh; Arata Kawashima; Miki Takahashi; Kayo Tomita; Kenji Kubota; Tetsuo Meshi; Satoshi Naito; Masayuki Ishikawa

doi:10.1128/jvi.76.5.2491-2497.2002

Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes

J Virol. 2002 Mar;76(5):2491-7. doi: 10.1128/jvi.76.5.2491-2497.2002.

Authors

Takuya Yamanaka¹, Takahiro Imai, Rena Satoh, Arata Kawashima, Miki Takahashi, Kayo Tomita, Kenji Kubota, Tetsuo Meshi, Satoshi Naito, Masayuki Ishikawa

Affiliation

¹ Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.

Abstract

The TOM1 gene of Arabidopsis thaliana encodes a putative multipass transmembrane protein which is necessary for the efficient multiplication of tobamoviruses. We have previously shown that mutations severely destructive to the TOM1 gene reduce tobamovirus multiplication to low levels but do not impair it completely. In this report, we subjected one of the tom1 mutants (tom1-1) to another round of mutagenesis and isolated a new mutant which did not permit a detectable level of tobamovirus multiplication. In addition to tom1-1, this mutant carried a mutation referred to as tom3-1. Positional cloning showed that TOM3 was one of two TOM1-like genes in Arabidopsis. Based on the similarity between the amino acid sequences of TOM1 and TOM3, together with the results of a Sos recruitment assay suggesting that both TOM1 and TOM3 bind tobamovirus-encoded replication proteins, we propose that TOM1 and TOM3 play parallel and essential roles in the replication of tobamoviruses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / virology*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Arabidopsis Proteins / pharmacology
Carrier Proteins / chemistry
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Carrier Proteins / pharmacology
Genetic Complementation Test
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Membrane Proteins / pharmacology
Molecular Sequence Data
Mutation*
Plant Diseases / virology
Plant Proteins / chemistry
Plant Proteins / genetics*
Plant Proteins / metabolism
Plant Proteins / pharmacology
Tobamovirus / genetics
Tobamovirus / pathogenicity*
Tobamovirus / physiology
Viral Proteins*
Virus Replication / drug effects*

Substances

Arabidopsis Proteins
Carrier Proteins
Membrane Proteins
Plant Proteins
TOM1 protein, Arabidopsis
TOM3 protein, Arabidopsis
Viral Proteins

Associated data

GENBANK/AB036427
GENBANK/AB057678