Abstract
The expression of class I patatin genes is restricted to potato tubers but can be induced in other tissues by exogenous sucrose. Here we show that tuber-specific and sucrose-inducible gene expression is reduced in transgenic potato plants by mutations in a conserved 10 base pair motif within the B-box of the patatin promoter. In a southwestern screen, we have isolated a novel DNA-binding protein designated Storekeeper (STK) that specifically recognises the B-box motif in vitro. Gel shift experiments with an STK-specific antibody suggest that STK is the B-box binding protein found in tuber nuclei. We propose that STK, the defining member of a new class of DNA binding proteins, regulates patatin expression in potato tubers via the B-box motif.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites / genetics
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Carboxylic Ester Hydrolases / genetics*
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Cloning, Molecular
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DNA Footprinting / methods
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DNA-Binding Proteins / genetics*
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Electrophoretic Mobility Shift Assay
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Gene Expression Regulation, Plant
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Molecular Sequence Data
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Mutation
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Plant Leaves / genetics
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Plant Proteins / genetics*
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Plant Stems / genetics
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Plants, Genetically Modified
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Promoter Regions, Genetic / genetics
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Sequence Homology, Amino Acid
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Signal Transduction
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Solanum tuberosum / genetics*
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Species Specificity
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Sucrose / pharmacology
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Transcription Factors / genetics
Substances
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DNA-Binding Proteins
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Plant Proteins
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RNA, Plant
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Transcription Factors
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patatin protein, Solanum tuberosum
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Sucrose
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Carboxylic Ester Hydrolases