Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis

Lílian Carla Carneiro; Fabrícia P de Faria; M Sueli S Felipe; Maristela Pereira; Célia M de Almeida Soares

doi:10.1016/j.fgb.2004.10.003

Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis

Fungal Genet Biol. 2005 Jan;42(1):51-60. doi: 10.1016/j.fgb.2004.10.003.

Authors

Lílian Carla Carneiro¹, Fabrícia P de Faria, M Sueli S Felipe, Maristela Pereira, Célia M de Almeida Soares

Affiliation

¹ Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.

PMID: 15588996
DOI: 10.1016/j.fgb.2004.10.003

Abstract

A proteomic approach was used to identify a 39 kDa antigen of Paracoccidioides brasiliensis. Amino acid sequences of the N-terminal and of endoproteinase Lys-C digested peptides revealed the protein to be a fructose 1,6-biphosphate aldolase (FBA) Class II of P. brasiliensis. Two cDNA homologues, Pbfba1 and Pbfba2, were cloned and characterized. Pbfba1 encoded a predicted polypeptide of 360 amino acids that was highly homologous in the primary structure to the same enzyme from fungi and bacteria. The other DNA, Pbfba2, encoded a polypeptide predicted to be 363 amino acids. The sequence of Pbfba2 differed significantly from Pbfba1. Phylogenetic and molecular analysis supports the concept of gene duplication for FBAs in P. brasiliensis, constituting a two-member family. Expression analysis demonstrated differential expression for both fbas genes in P. brasiliensis cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / isolation & purification
DNA, Fungal* / chemistry
DNA, Fungal* / genetics
DNA, Fungal* / isolation & purification
Fructose-Bisphosphate Aldolase / genetics*
Fructose-Bisphosphate Aldolase / isolation & purification
Fructose-Bisphosphate Aldolase / metabolism
Fungal Proteins / genetics
Fungal Proteins / isolation & purification
Gene Duplication
Molecular Sequence Data
Open Reading Frames
Paracoccidioides / enzymology*
Paracoccidioides / genetics
Phylogeny
Proteome / analysis
Sequence Analysis, DNA
Sequence Analysis, Protein
Sequence Homology, Amino Acid

Substances

DNA, Complementary
DNA, Fungal
Fungal Proteins
Proteome
Fructose-Bisphosphate Aldolase

Associated data

GENBANK/AY057387
GENBANK/AY172327
GENBANK/AY233454
GENBANK/AY581212