Abstract
A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular*
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Culture Media
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Gluconobacter oxydans / enzymology*
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Gluconobacter oxydans / genetics
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Molecular Sequence Data
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NADP / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Substrate Specificity
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Sugar Alcohol Dehydrogenases / chemistry
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Sugar Alcohol Dehydrogenases / genetics*
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Sugar Alcohol Dehydrogenases / metabolism*
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Sugar Alcohols / metabolism
Substances
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Culture Media
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Sugar Alcohols
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NADP
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Sugar Alcohol Dehydrogenases
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D-arabinitol dehydrogenase
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arabitol