A TRAP transporter for pyruvate and other monocarboxylate 2-oxoacids in the cyanobacterium Anabaena sp. strain PCC 7120

J Bacteriol. 2010 Nov;192(22):6089-92. doi: 10.1128/JB.00982-10. Epub 2010 Sep 17.

Abstract

In the cyanobacterium Anabaena sp. strain PCC 7120, open reading frames (ORFs) alr3026, alr3027, and all3028 encode a tripartite ATP-independent periplasmic transporter (TRAP-T). Wild-type filaments showed significant uptake of [(14)C]pyruvate, which was impaired in the alr3027 and all3028 mutants and was inhibited by several monocarboxylate 2-oxoacids, identifying this TRAP-T system as a pyruvate/monocarboxylate 2-oxoacid transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / metabolism*
  • Carbon Radioisotopes / metabolism
  • Carboxylic Acids / metabolism
  • Enzyme Inhibitors / metabolism
  • Gene Deletion
  • Membrane Transport Proteins / metabolism*
  • Mutagenesis, Insertional
  • Pyruvic Acid / metabolism*
  • Staining and Labeling

Substances

  • Carbon Radioisotopes
  • Carboxylic Acids
  • Enzyme Inhibitors
  • Membrane Transport Proteins
  • Pyruvic Acid