Novel (2R,3R)-2,3-butanediol dehydrogenase from potential industrial strain Paenibacillus polymyxa ATCC 12321

Bo Yu; Jibin Sun; Rajesh Reddy Bommareddy; Lifu Song; An-Ping Zeng

doi:10.1128/AEM.02998-10

Novel (2R,3R)-2,3-butanediol dehydrogenase from potential industrial strain Paenibacillus polymyxa ATCC 12321

Appl Environ Microbiol. 2011 Jun;77(12):4230-3. doi: 10.1128/AEM.02998-10. Epub 2011 Apr 29.

Authors

Bo Yu¹, Jibin Sun, Rajesh Reddy Bommareddy, Lifu Song, An-Ping Zeng

Affiliation

¹ Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21071 Hamburg, Germany.

Abstract

A (2R,3R)-2,3-butanediol dehydrogenase (BDH99::67) from Paenibacillus polymyxa ATCC 12321 was functionally characterized. The genetic characteristics of BDH99::67 are completely different from those of meso- and (2S,3S)-2,3-butanediol dehydrogenases. The results showed that BDH99::67 belongs to the medium-chain dehydrogenase/reductase superfamily and not to the short-chain dehydrogenase/reductase superfamily, to which meso- and (2S,3S)-2,3-butanediol dehydrogenases belong.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Oxidoreductases / genetics*
Alcohol Oxidoreductases / metabolism*
Butylene Glycols / metabolism*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Kinetics
Molecular Sequence Data
Paenibacillus / enzymology*
Phylogeny
Sequence Analysis, DNA
Sequence Homology

Substances

Butylene Glycols
DNA, Bacterial
2,3-butylene glycol
Alcohol Oxidoreductases
butanediol dehydrogenase

Associated data

GENBANK/HQ730089
GENBANK/JF440646