Abstract
We identify an Arabidopsis pyridoxal-phosphate-dependent aminotransferase, VAS1, whose loss-of-function simultaneously increases amounts of the phytohormone auxin and the ethylene precursor 1-aminocyclopropane-1-carboxylate. VAS1 uses the ethylene biosynthetic intermediate methionine as an amino donor and the auxin biosynthetic intermediate indole-3-pyruvic acid as an amino acceptor to produce L-tryptophan and 2-oxo-4-methylthiobutyric acid. Our data indicate that VAS1 serves key roles in coordinating the amounts of these two vital hormones.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acids, Cyclic / metabolism
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Ethylenes / biosynthesis*
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Indoleacetic Acids / metabolism*
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Indoles
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Methionine / analogs & derivatives
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Methionine / biosynthesis
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Methionine / metabolism
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Molecular Sequence Data
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Mutation
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Pyridoxal Phosphate / metabolism
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Transaminases / genetics
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Transaminases / metabolism*
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Tryptophan / biosynthesis
Substances
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Amino Acids, Cyclic
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Arabidopsis Proteins
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Ethylenes
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Indoleacetic Acids
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Indoles
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1-aminocyclopropane-1-carboxylic acid
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indol-3-yl pyruvic acid
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2-keto-4-methylthiobutyric acid
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Pyridoxal Phosphate
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Tryptophan
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ethylene
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Methionine
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VAS1 protein, Arabidopsis
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Transaminases
Associated data
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GENBANK/ACU18134
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GENBANK/ACU20038
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RefSeq/NP_001042188
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RefSeq/NP_178152
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RefSeq/XP_001690253
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RefSeq/XP_001753569
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RefSeq/XP_002284514
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RefSeq/XP_002284955
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RefSeq/XP_002299622
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RefSeq/XP_002517536
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RefSeq/XP_002887833