Coordination of auxin and ethylene biosynthesis by the aminotransferase VAS1

Nat Chem Biol. 2013 Apr;9(4):244-6. doi: 10.1038/nchembio.1178. Epub 2013 Feb 3.

Abstract

We identify an Arabidopsis pyridoxal-phosphate-dependent aminotransferase, VAS1, whose loss-of-function simultaneously increases amounts of the phytohormone auxin and the ethylene precursor 1-aminocyclopropane-1-carboxylate. VAS1 uses the ethylene biosynthetic intermediate methionine as an amino donor and the auxin biosynthetic intermediate indole-3-pyruvic acid as an amino acceptor to produce L-tryptophan and 2-oxo-4-methylthiobutyric acid. Our data indicate that VAS1 serves key roles in coordinating the amounts of these two vital hormones.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids, Cyclic / metabolism
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Ethylenes / biosynthesis*
  • Indoleacetic Acids / metabolism*
  • Indoles
  • Methionine / analogs & derivatives
  • Methionine / biosynthesis
  • Methionine / metabolism
  • Molecular Sequence Data
  • Mutation
  • Pyridoxal Phosphate / metabolism
  • Transaminases / genetics
  • Transaminases / metabolism*
  • Tryptophan / biosynthesis

Substances

  • Amino Acids, Cyclic
  • Arabidopsis Proteins
  • Ethylenes
  • Indoleacetic Acids
  • Indoles
  • 1-aminocyclopropane-1-carboxylic acid
  • indol-3-yl pyruvic acid
  • 2-keto-4-methylthiobutyric acid
  • Pyridoxal Phosphate
  • Tryptophan
  • ethylene
  • Methionine
  • VAS1 protein, Arabidopsis
  • Transaminases

Associated data

  • GENBANK/ACU18134
  • GENBANK/ACU20038
  • RefSeq/NP_001042188
  • RefSeq/NP_178152
  • RefSeq/XP_001690253
  • RefSeq/XP_001753569
  • RefSeq/XP_002284514
  • RefSeq/XP_002284955
  • RefSeq/XP_002299622
  • RefSeq/XP_002517536
  • RefSeq/XP_002887833