Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance

FEMS Microbiol Lett. 2015 Jan;362(3):1-7. doi: 10.1093/femsle/fnu042. Epub 2014 Dec 4.

Abstract

Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.

Keywords: Acs2; Saccharomyces cerevisiae; acetic acid; acetyl-CoA synthetase; lignocellulose.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetate-CoA Ligase / genetics*
  • Acetate-CoA Ligase / metabolism*
  • Acetic Acid / metabolism*
  • Acetyl Coenzyme A / metabolism
  • Fermentation
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • Plasmids
  • Real-Time Polymerase Chain Reaction
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / physiology*

Substances

  • Acetyl Coenzyme A
  • Acetate-CoA Ligase
  • Acetic Acid