Abstract
Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation.
MeSH terms
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Amino Acid Sequence
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Apoptosis / drug effects
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Blotting, Western
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Cell Cycle / drug effects
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Cell Proliferation / drug effects*
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Cells, Cultured
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Cloning, Molecular*
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Dictyostelium / enzymology*
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Gene Expression Regulation / physiology*
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Humans
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Immunoenzyme Techniques
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Molecular Sequence Data
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Ornithine / metabolism*
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Ornithine Decarboxylase / chemistry
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Ornithine Decarboxylase / genetics*
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Ornithine Decarboxylase / metabolism
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Phylogeny
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Protein Conformation
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Putrescine / pharmacology
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
Substances
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RNA, Messenger
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Ornithine
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Ornithine Decarboxylase
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Putrescine