Improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus Phanerochaete sordida YK-624

Jianqiao Wang; Sho Hirabayashi; Toshio Mori; Hirokazu Kawagishi; Hirofumi Hirai

doi:10.1016/j.jbiosc.2015.12.002

Improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus Phanerochaete sordida YK-624

J Biosci Bioeng. 2016 Jul;122(1):17-21. doi: 10.1016/j.jbiosc.2015.12.002. Epub 2016 Jan 4.

Authors

Jianqiao Wang¹, Sho Hirabayashi², Toshio Mori¹, Hirokazu Kawagishi³, Hirofumi Hirai⁴

Affiliations

¹ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
² Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
³ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
⁴ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.

PMID: 26766784
DOI: 10.1016/j.jbiosc.2015.12.002

Abstract

To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.

Keywords: Ethanol production; Phanerochaete sordida YK-624; Pyruvate decarboxylase; Recombinant expression; White-rot fungi.

MeSH terms

Bioreactors
Ethanol / metabolism*
Fermentation*
Metabolic Engineering
Phanerochaete / genetics*
Phanerochaete / metabolism*
Pyruvate Decarboxylase / genetics*
Pyruvate Decarboxylase / metabolism*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

Recombinant Proteins
Ethanol
Pyruvate Decarboxylase