Abstract
The nucleotide (nt) sequence of the bpdC1C2BADE genes which encode the first three enzymes in the biphenyl (BP) degradation pathway of Gram+ Rhodococcus sp. M5 (formerly Arthrobacter M5) was determined. Except for the ferredoxin component (BpdB) of the initial BP dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (TodC1C2BADE) present in the toluene-degrader, Pseudomonas putida F1, than those of three BP-degrading pseudomonads. The cloned bpd genes were verified by their expression in the Escherichia coli T7 RNA polymerase/promoter system. In E. coli, BpdA was able to complement TodC1C2B in indigo biosynthesis, although the M5 native or cloned BP dioxygenase does not carry out this reaction.
MeSH terms
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Bacterial Proteins / genetics*
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Biodegradation, Environmental
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Biphenyl Compounds / metabolism*
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Cloning, Molecular
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DNA, Ribosomal / genetics
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Escherichia coli / genetics
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Genes, Bacterial*
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Genetic Complementation Test
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Iron-Sulfur Proteins*
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Molecular Sequence Data
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Oxygenases / genetics
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Oxygenases / metabolism
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Polychlorinated Biphenyls / metabolism*
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RNA, Ribosomal, 16S / genetics
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Recombinant Proteins / metabolism
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Rhodococcus / classification
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Rhodococcus / enzymology
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Rhodococcus / genetics*
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Rhodococcus / metabolism
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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Biphenyl Compounds
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DNA, Ribosomal
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Iron-Sulfur Proteins
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RNA, Ribosomal, 16S
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Recombinant Proteins
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diphenyl
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Polychlorinated Biphenyls
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Oxygenases
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biphenyl-2,3-dioxygenase
Associated data
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GENBANK/U27579
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GENBANK/U27591
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GENBANK/X77779
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GENBANK/X77780