Abstract
Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pts deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the glpK gene that could encode regions important for the activity of glycerol kinase. The results strongly suggest that the main effect of the PTS on glycerol utilization in B. subtilis is mediated via glycerol kinase.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology
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Bacillus subtilis / genetics*
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Bacillus subtilis / growth & development
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Base Sequence
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Binding Sites
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DNA Primers
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Escherichia coli / enzymology
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Gene Deletion*
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Genes, Bacterial
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Genes, Suppressor
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Genotype
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Glycerol / metabolism*
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Glycerol Kinase / chemistry
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Glycerol Kinase / genetics*
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Glycerol Kinase / metabolism
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Models, Structural
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Molecular Sequence Data
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Phenotype
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Plasmids
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Polymerase Chain Reaction
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Protein Conformation
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Species Specificity
Substances
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DNA Primers
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Glycerol Kinase
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Glycerol