The nucleotide sequence of genes involved in the leucine biosynthetic pathway of Clostridium pasteurianum

DNA Seq. 1993;4(2):105-11. doi: 10.3109/10425179309020149.

Abstract

A 2.2 kb SphI/ClaI fragment of the Clostridium pasteurianum chromosome has previously been cloned and shown to complement leuB401 and leuC171 mutations in Escherichia coli. The nucleotide sequence of this fragment has been determined (2327 bp) and carries three open reading frames. The products of translation of these reading frames display significant homologies with the alpha-isopropylmalate isomerase subunit (leuD) gene of Salmonella typhimurium, the beta-isopropylmalate dehydrogenase (leuB) genes of several organisms, and the dihydroxyacid dehydrase (ilvD) gene of E. coli.

Publication types

  • Comparative Study

MeSH terms

  • 3-Isopropylmalate Dehydrogenase
  • Alcohol Oxidoreductases / genetics
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosomes, Bacterial
  • Clostridium / enzymology*
  • Clostridium / genetics*
  • Consensus Sequence
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Genes, Bacterial*
  • Hydro-Lyases / genetics*
  • Leucine / biosynthesis*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Open Reading Frames
  • Phylogeny
  • Point Mutation
  • Restriction Mapping
  • Salmonella typhimurium / enzymology
  • Salmonella typhimurium / genetics
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Macromolecular Substances
  • Alcohol Oxidoreductases
  • 3-Isopropylmalate Dehydrogenase
  • Hydro-Lyases
  • 3-isopropylmalate dehydratase
  • leuD protein, Bacteria
  • dihydroxyacid dehydratase
  • Leucine

Associated data

  • GENBANK/L06666